Cloning, sequencing, and regulation of a xylanase gene from the fungus Aureobasidium pullulans Y-2311-1
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چکیده
منابع مشابه
Purification and characterization of a new xylanase (APX-II) from the fungus Aureobasidium pullulans Y-2311-1.
Aureobasidium pullulans Y-2311-1 produced four major xylanases (EC 3.2.1.8) with pI values of 4.0, 7.3, 7.9, and 9.4 as revealed by isoelectric focusing and zymogram analysis when grown for 4 days on 1.0% oat spelt xylan. The enzyme with a pI of 9.4 was purified by ammonium sulfate precipitation, chromatography on a DEAE-Sephadex A-50 column, and gel filtration with a Sephadex G-75 column. The ...
متن کاملIDENTIFICATION, ISOLATION, CLONING AND SEQUENCING APARTIALANNEXIN GENE FROM AUREOBASIDIUM PULLULANS
Background and Objectives: Annexin is the common name for genes and proteins that were identified as calcium-dependent phospholipid-binding proteins. Recently a more complex set of functions has been recognized for this superfamily of proteins including in vesicle trafficking, cell division, apoptosis, calcium signalling, mineralization, crystal nucleation inside the extracellular organelle...
متن کاملidentification, isolation, cloning and sequencing apartialannexin gene from aureobasidium pullulans
background and objectives: annexin is the common name for genes and proteins that were identified as calcium-dependent phospholipid-binding proteins. recently a more complex set of functions has been recognized for this superfamily of proteins including in vesicle trafficking, cell division, apoptosis, calcium signalling, mineralization, crystal nucleation inside the extracellular organelles-ma...
متن کاملPartial Optimization of Endo-1, 4-Β-Xylanase Production by Aureobasidium pullulans Using Agro-Industrial Residues
UNLABELLED Objective(s) : Although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed. MATERIALS AND METHODS Single xylanase producer yeast was isolated from plant residues based on formation of transparent halo zones on xylan agar plates. The isolate showed much greate...
متن کاملAntibacterial Activity of Aureobasidium Pullulans
Aureobasidium pullulans is ubiquitous, polymorphic and black fungus producing a variety of important metabolites including enzymes, polysaccharides and single cell protein. It is considered as a probable source for antagonistic activity against a number of phytopathogenic fungi and possible biocontrol agent of post harvest diseases. The present work aims at the antibacterial compounds produced ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1994
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.60.12.4647-.1994